Genetics · 2001 · 851 citations · 46 references
Life SciencesGeneticsMolecular BiologyGene ExpressionGene RegulationTranscriptional RegulationBacterial Gene RegulationGene Regulation In MicrobesGene Regulatory ElementsTranscriptional Regulation In EukaryotesTranscriptional Regulation In ProkaryotesUltraviolet Radiation BiologyBacterial Open Reading FramesCellular RecoveryGene Regulation In BacteriaLight-inducible Gene ExpressionRadiation Response Gene ExpressionRadiation-induced Gene ExpressionTranscriptsUv Stress Response
The SOS response in UV-irradiated Escherichia coli includes the upregulation of several dozen genes that are negatively regulated by the LexA repressor. Using DNA microarrays containing amplified DNA fragments from 95.5% of all open reading frames identified on the E. coli chromosome, we have examined the changes in gene expression following UV exposure in both wild-type cells and lexA1 mutants, which are unable to induce genes under LexA control. We report here the time courses of expression of the genes surrounding the 26 documented lexA-regulated regions on the E. coli chromosome. We observed 17 additional sites that responded in a lexA-dependent manner and a large number of genes that were upregulated in a lexA-independent manner although upregulation in this manner was generally not more than twofold. In addition, several transcripts were either downregulated or degraded following UV irradiation. These newly identified UV-responsive genes are discussed with respect to their possible roles in cellular recovery following exposure to UV irradiation.
46
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
Mark Schena, Dari Shalon, Ronald W. Davis et al. · Science · 1995 · 9.6K citations
The Complete Genome Sequence of <i>Escherichia coli</i> K-12
Frederick R. Blattner, Guy Plunkett, Craig A. Bloch et al. · Science · 1997 · 7.5K citations
D. Bootsma, Jan H.J. Hoeijmakers · Trends in Genetics · 1996 · 2.3K citations