Fabrication of an Oriented Fc‐Fused Lectin Microarray through Boronate Formation

Abstract

A stable, covalent, and highly active protein microarray was created through the formation of cyclic esters between surface boronic acids and the carbohydrate moiety of a fusion protein, Fc-dectin-1 (see picture). A biotin-labeled polysaccharide was used as a probe to investigate the binding activity of the protein. Staining of the product array with streptavidin-Cy3 revealed the effectiveness of this immobilization strategy.