Chemistry - A European Journal · 2009 · 31 citations · 30 references
Natural SciencesCell BiologyMedicinal ChemistryBiomedical ImagingBiomedical OpticsMolecular ImagingCellular ImagingFluorescence ImagingFluorescence MicroscopyFluorescence SpectroscopyMolecular Probe DevelopmentPhotophysics Of Fluorescent ProbesCellular Fluorescence ImagingExtinction CoefficientsFluorescence Imaging ProbesFluorescence Quantum YieldFluorophore DesignLive-cell Fluorescence ImagingLive-cell Imaging ProbesMolar Extinction Coefficient
We present the synthesis and photophysical characterisation of a series of structurally diverse, fluorescent 2,6,8-trisubstituted 3-hydroxychromone derivatives with high fluorescence quantum yields and molar extinction coefficients. Two of these derivatives (9 and 10 a) have been studied as fluorophores for cellular imaging in HeLa cells and show excellent permeability and promising fluorescence properties in a cellular environment. In addition, we have demonstrated by photophysical characterisation of 3-isobutyroxychromone derivatives that esterification of the 3-hydroxyl group results in acceptable and useful fluorescence properties.
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