Genomic instability in colorectal cancer: relationship to clinicopathological variables and family history.

TLDR

Recent studies indicate that replication errors caused by gene mutations and additional alleles at microsatellite loci contribute to colorectal tumorigenesis. The authors examined seven dinucleotide repeat loci on different chromosomes in colorectal tumors using PCR amplification and denaturing PAGE, and also tested the same markers in breast and male germ cell cancers. In 243 colorectal cancers, 16.5 % exhibited replication errors at one or more loci, with higher rates in families with a strong history (31 %) versus sporadic cases (17 %); RER⁺ tumors were associated with DNA diploid status, proximal colon location, and poor differentiation, and patients with RER⁺ at ≥2 loci had improved survival, while no RER⁺ tumors were found in breast or germ cell cancers.

Abstract

Recent reports have suggested that one or more genes may cause replication errors (RER) during colorectal tumorigenesis. Additional alleles are seen in the tumors when analyzing random microsatellite loci. We have studied seven dinucleotide repeat loci, located on seven different chromosomes, by use of polymerase chain reaction amplification and denaturing polyacrylamide gel electrophoresis. We found that 16.5% (40 of 243) colorectal cancers showed RER at one or several loci (RER+). This includes 31% (4 of 13) among cases with a strong positive family history according to previously published criteria and 17% (35 of 207) among cases with no history of familial cancer. Interestingly, no significant association was found between RER+ tumors and a general familial clustering of cancer. Microsatellite instability was significantly associated with DNA diploid status of the tumor (P < 0.001), with the location of the tumor in the proximal colon (P < 0.001), and with poorly differentiated tumor phenotype (P < 0.001). Patients with RER+ at > or = 2 loci tumors had an increased survival (P = 0.05). We further analyzed 84 breast cancers and 86 male germ cell cancers using the same seven markers. None of the tumors were RER+, indicating that this phenomenon may be specific to certain types of tumors.