Characterization and Antagonistic Activities of Metabolite Produced by Pseudomonas Aeruginosa Sha8

Abstract

The antagonistic activities of Bacillus firmus and Pseudomonas aeruginosa which were isolated from rhizosphere field of Triticum sativum cultivated in El-Sharkia governorate, Egypt; were studied against 13 fungal isolates. The optimum conditions for growth and maximum production of antifungal activity were detected against Aspergillus niger, Helminthosporium sp. and Fusarium oxysporium. Only P. aeruginosa was chosen for more investigation as antifungal agent. King's B medium was used for production of the antifungal metabolite which extracted by hexane. The extracted metabolite appeared as one spot on TLC and was characterized by using infra red spectrum (IR), nuclear magnetic resonance (NMR), mass spectrum (MS) and elemental analysis were detected. The compound was considered as a derivative of phenazine-1-caboxamide PCN (zag1).The minimum inhibitory concentration of this compound against A. niger was 23.55µg/ml. The antifungal activity of this compound was remarkable at 100°C for 30 mins. Different morphological and ultrastructure changes induced after treatment of A. niger by 23.55µg/ml PCN (zag1) were recorded by light and transmission electron microscope.