The modification of siRNA with 3′ cholesterol to increase nuclease protection and suppression of native mRNA by select siRNA polyplexes

Analyzing real-time PCR data by the comparative CT method

Thomas D. Schmittgen, Kenneth J. Livak · Nature Protocols · 2008 · 26K citations

Computed Tomography, Image Analysis, Engineering +8

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

Sayda M. Elbashir, Jens Harborth, Winfried Lendeckel et al. · Nature · 2001 · 9.3K citations

Natural Sciences, Rna Splicing, Rna Biology +9

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

Otmane Boussif, Frank Lezoualc’h, Maria Antonietta Zanta et al. · Proceedings of the National Academy of Sciences · 1995 · 6.2K citations · Full text

Engineering, In Vivo Gene Therapy, Several Polycations +20

Role for a bidentate ribonuclease in the initiation step of RNA interference

Emily Bernstein, Amy A. Caudy, Scott M. Hammond et al. · Nature · 2001 · 4.9K citations

Rna Interference, Genetics, Natural Sciences +12

A System for Stable Expression of Short Interfering RNAs in Mammalian Cells

Thijn R. Brummelkamp, René Bernards, Reuven Agami · Science · 2002 · 4.3K citations · Full text

Functional Genomics, Psuper Vector, Short Interfering Rnas +15