Cytogenetic and Genome Research · 2014 · 50 citations · 35 references
GeneticsCell CycleGenomicsCentromeric ChromatinEpigeneticsHistone VariantsPlant Molecular BiologyAnti-phosphorylated Histone H2athr120Plant CytologyUniversal Microscopic MarkerCell DivisionChromatin BiologyNuclear OrganizationHistone H2aCell BiologyChromatin FunctionBiologyChromatinChromatin StructureChromatin RemodelingNatural SciencesEpigenomicsChromosome BiologyMedicine
Based on the analysis of 20 different monocot and eudicot species, we propose that the centromeric distribution of the phosphorylated histone H2AThr120 is evolutionary highly conserved across species with mono- and holocentric chromosomes. Therefore, antibodies recognizing the phosphorylated threonine 120 of the histone H2A can serve as a universal marker for the cytological detection of centromeres of mono- and holokinetic plant species. In addition, super resolution microscopy of signals specific to the centromere-specific histone H3 variant CENH3 and to H2AThr120ph revealed that these histone variants are incorporated into different nucleosomes, which form distinct, partly intermingled chromatin domains. This specific arrangement of both histone variants suggests different centromeric functions during the cell cycle.
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Autoantibody to centromere (kinetochore) in scleroderma sera.
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