High Thermal Stability of 5′‐5′‐Linked Alternate Hoogsteen Triplexes at Physiological pH

Abstract

DNA glue: A new alternate-strand linker (shown in green) has been synthesized that can connect two short DNA sequences (5- and 9-mers, yellow) and bind to double-stranded DNA through major-groove interactions at pH 7.2 (see model). The resulting triple helices have high thermal stabilities as shown by melting temperatures exceeding 37 °C at physiological pH values. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z601127_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.