Urease immobilized on aminated butyl acrylate‐ethylenedimethacrylate copolymer

Abstract

Abstract Urease was covalently immobilized on a new acrylic enzyme carrier: aminated butyl acrylate‐ethylenedimethacrylate copolymer. The enzyme retained 56% of its original activity. The properties of the free and immobilized urease were determined and compared. The Michaelis constant was found to be higher for the immobilized urease than for the free one, while maximum reaction rate was lower for the immobilized enzyme. The stability of urease against change in pH was considerably improved by the immobilization. The immobilized urease showed very good storage stability and reusability. Resistance of the enzyme against heat inactivation at 70°C, however, was not found to be improved.