Journal of Bioactive and Compatible Polymers · 2005 · 153 citations · 10 references
Tissue EngineeringEngineeringBiomaterials DesignBiofabricationBiomedical EngineeringOrgan RegenerationOrgan Manufacturing TechniqueRegenerative MedicineHydrogelsBiomaterial ModelingMatrix BiologyBiophysicsThree-dimensional StructureFunctional Tissue EngineeringCell EngineeringCell Biology3D Bioprinting3D PrintingBiopolymer GelVivo GrowthSelf-assemblyOrganoidsTissue CultureMedicineBiomaterialsHuman TissueExtracellular Matrix
An organ manufacturing technique was developed that enables the formation of cell/extracellular matrix (ECM) complexes for in vitro or in vivo growth. In this study, a three-dimensional (3D) structure composed of hepatocytes and gelatin/alginate hydrogel was made using a cell assembler-I apparatus to thoroughly control cell assembling. Hepatocytes and ECM were constructed into 10 X 10 X 3mm 3 structures according to a designed pattern. The embedded hepatocytes remained viable and performed biological functions in the construct for more than 12 days. This 3D structure has the potential to be used as a precursor for tissue or organ regeneration. This technology offers the potential for high-throughput production of artificial human tissues and organs.
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Organ printing: computer-aided jet-based 3D tissue engineering
Vladimir Mironov, Thomas Boland, Thomas C. Trusk et al. · Trends in biotechnology · 2003 · 1.3K citations