Response of lactating goats to low phosphorus intake 2. Nitrogen transfer from rumen ammonia to rumen microbes and proportion of milk protein derived from microbial amino acids

Abstract

Summary Five rumen-cannulated dairy goats were fed diets supplying 2·8 g P/day plus 1·4 g P/kg milk (adequate P) for weeks 1–6 of lactation. Two goats then continued to receive this adequate P supply while three goats were fed deficient diets supplying only 0·8 g P/day plus 0·4 g P/kg milk. Solutions containing CrEDTA and ( 15 NH 4 ) 2 SO 4 were infused into the rumen continuously for 96 h during weeks 5–6 and 11–12, respectively. At intervals following the intraruminal infusion, 15 N enrichments of rumen ammonia-N, rumen bacteria-N and milk protein-N were determined. Cr concentration was measured in particle-free rumen fluid. P deficiency did not significantly affect rumen fluid kinetics. It caused a significant increase in pH and reduced the size of the rumen ammonia pool and its outflow rate. Digestibility of organic matter as well as efficiency of microbial protein synthesis were decreased significantly and thus net microbial yield was reduced from 34·1 to 13·7 g N/day. The transfer of N of microbial origin to milk protein decreased from 5·3 to 2·7 g/day, whereas secretion of N in milk protein not originating from rumen microbes remained unchanged at 5·6–5·8 g/day.