Chemical Communications · 2012 · 52 citations · 29 references
Life SciencesChemistryMolecular BiologyAnalytical ChemistryNucleic Acid BiochemistryNucleic Acid ChemistryDna ChemistryNucleic Acid EngineeringPolymerase Chain ReactionAmplificationDna Polymerase ChemistryIonic LiquidsMild Reaction ConditionsPolymerase Chain Reaction (Pcr) Amplification
A bicyclic imidazolium ionic liquid (4d), [b-4C-im][Br], was found to be highly effective not only for promoting PCR of GC-rich DNA by minimizing non-specific amplification, but also for facilitating PCR of normal-GC DNA under mild conditions.
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Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Randall K. Saiki, David H. Gelfand, Susanne Stoffel et al. · Science · 1988 · 17.2K citations
Ralf Giernoth · Angewandte Chemie International Edition · 2010 · 737 citations
Betaine Improves the PCR Amplification of GC-Rich DNA Sequences
Wolfgang Henke, K. Herdel, Klaus Jung et al. · Nucleic Acids Research · 1997 · 523 citations · Full text