Concepedia

A FRET‐Based Fluorogenic Phosphine for Live‐Cell Imaging with the Staudinger Ligation

Matthew J. Hangauer, Carolyn R. Bertozzi

Angewandte Chemie International Edition · 2008 · 189 citations · 29 references

DOIFull text

Open access

Concepts

Abstract

Azide imaging: A fluorogenic phosphine based on a FRET-quenching mechanism allows for live-cell imaging of azido sugars by the Staudinger ligation. This design strategy can accommodate numerous fluorophores and complementary quenchers, enabling extension to multicolor imaging. In the image shown, the nuclei are blue while the cell surfaces and Golgi are green.

References

29

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Vsevolod V. Rostovtsev, Luke G. Green, Valery V. Fokin et al. · Angewandte Chemie International Edition · 2002 · 11.5K citations

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Peptidotriazoles on Solid Phase:  [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides

Christian W. Tornøe, Caspar Christensen, Morten Meldal · The Journal of Organic Chemistry · 2002 · 8.5K citations

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A Strain-Promoted [3 + 2] Azide−Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems

Nicholas J. Agard, Jennifer A. Prescher, Carolyn R. Bertozzi · Journal of the American Chemical Society · 2004 · 2.9K citations

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Cell Surface Engineering by a Modified Staudinger Reaction

Eliana Saxon, Carolyn R. Bertozzi · Science · 2000 · 2.4K citations

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Copper-free click chemistry for dynamic <i>in vivo</i> imaging

Jeremy M. Baskin, Jennifer A. Prescher, Scott T. Laughlin et al. · Proceedings of the National Academy of Sciences · 2007 · 1.8K citations · Full text

, , +17