Plasmids for Nicotine-Dependent and -Independent Gene Expression in <i>Arthrobacter nicotinovorans</i> and Other <i>Arthrobacter</i> Species

Abstract

The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow the production of complex enzymes and genetic complementation studies in Arthrobacter species.