ChemPhysChem · 2006 · 16 citations · 20 references
Natural SciencesChemistryCrystal StructureSpectroscopyStructural BiologyCrystal ChemistryCrystallographyElectron Paramagnetic ResonanceElectron Spin Resonance SpectroscopyHyperfine StructureMagnetism In SolidsCoordination Compound MagnetismDeuterium ChemistryDeuterium Nuclear Magnetic ResonanceElectron DelocalizationParamagnetic CentersParamagnetic SpeciesSpin Densities In MoleculesSpin Density Distribution
A 95 GHz pulsed deuterium ENDOR study has been performed on single crystals of azurin from Pseudomonas aeruginosa selectively deuterated at the C(beta) position of the copper-coordinating cysteine 112. Complete hyperfine tensors of the two deuterium atoms have been obtained, which reveal identical isotropic parts. Analysis of the hyperfine tensors provides insight into the spin-density delocalization over the cysteine ligand. Approximately 45 % of the spin density in the paramagnetic site can be attributed to copper and 30 % to sulfur.
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