The hydrogen ion dissociation curve of the crystalline albumin of the hen's egg

Abstract

Tmis paper is a report upon a somewhat extensive investigation of the hydrogen electrode titration of crystalline egg albumin.The work was undertaken with the object of examining the limitations of the method as a means of establishing a quantitative definition of the amphoteric properties of a protein.Crystalline egg albumin was chosen because it is probably the best accredited example of a molecularly homogeneous protein.The extensive work of S0rensen and of his colleagues, of Svedberg and of others indicates that of the common protein preparations egg albumin exhibits the greatest degree of constancy in composition, molecular weight and solubility under varying conditions of preparation and treatment.This protein has the further advantage that it is soluble in water Commonwealth Fund Fellow.