A Population of Thermostable Reverse Transcriptases Evolved from <i>Thermus aquaticus</i> DNA Polymerase I by Phage Display

Abstract

Running in reverse: A set of enzymes selected according to their RNA-dependent DNA polymerase activity from a library of more than 107 mutant enzymes has been characterized. The product was specifically bound to catalytically active proteins displayed on filamentous bacteriophage. The selected variants have a catalytic efficiency for reverse transcription that is two orders of magnitude higher. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z601217_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.