Photochemistry and Photobiology · 1977 · 391 citations · 8 references
Natural SciencesBiophysicsChemistryAnalytical ChemistrySpectroscopic AnalysisSpectroscopyBiophysical SpectroscopyElectronic SpectroscopyEmission SpectroscopyFluorescence SpectroscopyMolecular SpectroscopyOptical SpectroscopyEmission Spectra AnalysisEmission SpectrumExcitation Spectra AnalysisFluorescence Emission SpectraFluorescence Excitation SpectraIndole ChemistryOptical FiltersPolarization
Abstract— The fluorescence excitation spectrum and the excitation polarization spectrum of indole in propylene glycol were measured at — 58°C, after selecting by optical filters the emission originating from the 1 L a electronic level. From the analysis of these spectra, the excitation spectrum was resolved into the 1 L a and 1 L a excitation bands. A similar resolution of the excitation spectrum of tryptophan is given. This method can also be applied to the resolution of the emission spectrum in cases of dual emission.
8