Efficient N‐Terminal Glycoconjugation of Proteins by the N‐End Rule

Abstract

Bulky amino acids in positions 2 and 3 of proteins protect both Met and noncanonical azidohomoalanine, introduced by the auxotrophy-based method, from being excised by the enzymes responsible for N-terminal Met excision. Bioorthogonal transformation enables new specific functionalization of target proteins. We validated this general concept by designing an N-terminal glycoconjugated barstar capable of lectin binding without losing its biological activity.