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The <i>Salmonella</i> Pathogenicity Island 2-Encoded Type III Secretion System Is Essential for the Survival of <i>Salmonella enterica</i> Serovar Typhimurium in Free-Living Amoebae

Benjamin Bleasdale, Penelope J. Lott, Aparna Jagannathan, Mark P. Stevens, Richard J. Birtles, Paul Wigley

Applied and Environmental Microbiology · 2009 · 43 citations · 21 references

Open access

Abstract

Free-living amoebae represent a potential reservoir and predator of Salmonella enterica. Through the use of type III secretion system (T3SS) mutants and analysis of transcription of selected T3SS genes, we demonstrated that the Salmonella pathogenicity island 2 is highly induced during S. enterica serovar Typhimurium infection of Acanthamoeba polyphaga and is essential for survival within amoebae.

References

21

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 176.9K citations

Functional Genomics, Engineering, Medicine +12

Unravelling the biology of macrophage infection by gene expression profiling of intracellular <i>Salmonella enterica</i>

Sofia Eriksson, Sacha Lucchini, Arthur R. Thompson et al. · Molecular Microbiology · 2002 · 847 citations · Full text

Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii

Michael Steinert, Levente Emödy, Rudolf Amann et al. · Applied and Environmental Microbiology · 1997 · 367 citations · Full text

Medical Microbiology, Legionella Pneumophila, Klebsiella Pneumoniae +11

During infection of epithelial cells Salmonella enterica serovar Typhimurium undergoes a time-dependent transcriptional adaptation that results in simultaneous expression of three type 3 secretion systems

Isabelle Hautefort, Arthur R. Thompson, Sofia Eriksson-Ygberg et al. · Cellular Microbiology · 2008 · 260 citations · Full text

Epithelial Cells Salmonella, Microbial Pathogens, Innate Immune System +19

Intracellular Growth in<i>Acanthamoeba castellanii</i>Affects Monocyte Entry Mechanisms and Enhances Virulence of<i>Legionella pneumophila</i>

Jeffrey D. Cirillo, Suat L. G. Cirillo, Ling Yan et al. · Infection and Immunity · 1999 · 237 citations · Full text