Phytotaxa · 2014 · 105 citations · 195 references
Life SciencesBotanyPlant BiologyBryologyMolecular EvolutionPhylogeneticsBryophyte EvolutionBryophyte GeneticsBryophyte PhylogenyBryophyte SystematicsBryophyte TaxonomyPlant PhylogenyDeep PhylogenyDna BarcodingMolecular Species DelimitationMolecular Species IdentificationPhylogenetic GenomicsPhylogenetic IdentificationPhylogenetic ReconstructionPhylogenetic Taxonomy
Reviews have tracked progress in bryophyte molecular phylogenetics, covering publication records, phylogeny, systematics, and marker evolution, but none have fully characterized all markers used. The study aims to overview the history and current state of marker utilization in bryophyte phylogenetics, covering coding, non‑coding, and fingerprinting markers. The authors outline the molecular architecture, evolutionary peculiarities, and practical amplification and sequencing strategies for commonly used DNA sequence markers across all three genomes. The review discusses the phylogenetic utility of these markers, their potential to address remaining questions in bryophyte phylogeny, and their suitability for DNA barcoding species identification.
A number of reviews have accompanied and monitored the progress of molecular phylogenetic research on bryophytes, focusing on the publication record itself, bryophyte phylogeny and systematics in the molecular era, as well as the evolution and phylogenetic utility of markers from different genomes. However, none of the recent reviews include a detailed characterization of all molecular markers used in bryophyte phylogenetics. Here we provide an overview of the history and current state of marker utilization, including coding and non-coding sequence markers from all three genomes as well as fingerprinting approaches. The molecular architecture and evolutionary peculiarities, as well as practical aspects such as amplification and sequencing strategies, are outlined for the DNA sequence markers, with a focus on the most commonly employed regions. Their phylogenetic utility and potential for solving some of the remaining, pressing questions in bryophyte phylogeny, as well as their suitability for molecular species identification by DNA barcoding, are discussed.
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Universal primers for amplification of three non-coding regions of chloroplast DNA
Pierre Taberlet, Ludovic Gielly, Guy Pautou et al. · Plant Molecular Biology · 1991 · 5.7K citations