Reverse Glycoblotting Allows Rapid‐Enrichment Glycoproteomics of Biopharmaceuticals and Disease‐Related Biomarkers

Abstract

Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).