Two‐Color Glycan Labeling of Live Cells by a Combination of Diels–Alder and Click Chemistry

Abstract

One is not enough: Terminal alkenes are used as chemical reporters and ligation partners for 1,2,4,5-tetrazines in a Diels–Alder reaction with inverse electron demand (DARinv). Combination with strain-promoted azide–alkyne cycloaddition (SPAAC) allows the visualization of two different glycan structures in one experiment.