Expression of outer membrane protein e of <i>escherichia coli</i> K12 by phosphate limitation

Abstract

Escherichia coli K12 contains several so-called&#13;\nmajor outer membrane proteins, the designation of&#13;\nwhich was different in various laboratories but it is now agreed that the proteins will be named&#13;\naccording to the designation of their structural gene. The products of the ompC (min 48) and ompF&#13;\n(min 21) genes, formerly designated as c and b in our&#13;\nlaboratory, are outer membrane proteins&#13;\nwhich function as aqueous pores through which many&#13;\nnutrients enter the cell via a diffusion-like process. Mutants which lack both the ompC and ompF&#13;\nproducts are sensitive to 3% sodium dodecyl sulphate&#13;\n(SDS)