In Vitro Propagation of Muscadine Grape by Axillary Shoot Proliferation

Abstract

Plantlets were recovered from axillary bud cultures of muscadine grape (Vitis rotundifolia, `Summit'). Nodal segments 0.5 to 1.0 cm long were cultured in Murashige and Skoog (MS) basal medium supplemented with 5, 10, 20, or 40 μ m BA. Best total shoot production was obtained with 10 μ m BA; with higher BA levels, shoots were unexpanded and exhibited high mortalities. MS medium supplemented with IBA enhanced rooting by increasing rooting percentage and number per plantlet. Shoots previously proliferated on medium with 5 μ m BA rooted significantly better than those multiplied on 10 μM BA. Shoot vigor during rooting was greater in shoots proliferated on 5 vs. 10 μ m BA. Root development was not significantly affected by liquid vs. agar-solidifted medium or shoot length. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA), 1H-indole-3-butyric acid (IBA).