Measuring rapid kinetics by a potentiometric method in droplet-based microfluidic devices

Abstract

Droplets containing RNA and Mg(2+) were generated in microfluidic channels. By integrating a group of pneumatic valves and phase separation channels in the microfluidic system, the rapid RNA-Mg(2+) binding kinetics was studied by measuring the Mg(2+) ion concentration using an ion-selective electrode.