An Integrated Self‐Assembled Nanofluidic System for Controlled Biological Chemistries

Abstract

Consecutive enzymatic reactions are executed by mixing attoliter volumes (released from nanometer-sized lipid vesicles) in a closed femtoliter reactor vessel (a larger unilamellar vesicle), thus controlling the number of reactants with single-molecule precision. The autonomous nanoreactors need no external interfacing such as outlets or inlets, allowing for extreme miniaturization. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.