Role of the Stationary Growth Phase Sigma Factor RpoS of <i>Burkholderia pseudomallei</i> in Response to Physiological Stress Conditions

Abstract

The Burkholderia pseudomallei rpoS gene was identified, and an rpoS null mutant was constructed. The mutant was shown to have an increased sensitivity to carbon starvation and oxidative stress. By using rpoS-lacZ fusions, transcription of rpoS was shown to be growth phase regulated, reaching a peak upon entry into stationary phase.