Development of a Two-Color Fluorescence In Situ Hybridization Technique for Species-Level Identification of Human-Infectious<i>Cryptosporidium</i>spp

Abstract

A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.