Quantitative Co-Expression of Proteins at the Single Cell Level – Application to a Multimeric FRET Sensor

Abstract

Quantitative co-expression of two or more proteins can be achieved with little cell-to-cell variability. This finding enables reliable co-expression of donor and acceptor tagged proteins for FRET studies, which is of particular importance for the development of novel bimolecular sensors that can be expressed from single plasmid.