<i>In Vitro</i> Detection of Transplantation Immunity: The Inhibition of Migration of Immune Spleen Cells and Peripheral Blood Leukocytes by Specific Antigen

Abstract

Abstract A modified capillary tube migration technique was used with rodent spleen cells and human peripheral blood leukocytes. Such cells were found to migrate in a consistent manner with the appropriate method. When cells were used from animal or human subjects sensitized to histocompatibility antigens and the corresponding lymphoid cells from the sensitizing strain or human donor were added to the culture media, after prior disruption by ultrasound, inhibition of migration was observed. This was consistent, immunologically specific and increased in degree with the state of immunity of the animal.