Label‐Free, Real‐Time Glycosyltransferase Assay Based on a Fluorescent Artificial Chemosensor

Abstract

Reality check: The fluorescent probe 1 is applied to a real-time glycosyltransferase assay. The fluorescence of 1 increases with the progress of the glycosyl transfer reaction. The high sensitivity and reliable output of the method under multisubstrate enzymatic conditions make it suitable for the high-throughput screening of glycosyltransferase inhibitors.