Cytochromes in Streptococcus faecalis var. zymogenes Grown in a Haematin-containing Medium

Abstract

Summary: Functional cytochromes were found in the membrane fraction of Streptococcus faecais var. zymogenes grown aerobically with haematin. Molar growth yields shovei that the cytochrome system produced additional ATP. Inhibitors and uneruglers of oxidative phosphorylation verified the presence of cytochromes in the membrane fraction. Spectra indicated both a and b type cytochromes when cultures were grown with haematin. Without haematin, only a flavin system of electron imsport developed without additional ATP generation. Bacteria grown anaeuoically with haematin did not form cytochromes.