Physiological, Biochemical, and Molecular Effects of In Vitro Induced Iron Deficiency in Peach Rootstock Mr.S 2/5

Abstract

Abstract On calcareous soils, carbonate induced iron deficiency can have heavy effects on growth and development of several fruit crops. Leaf chlorosis, biochemical, and ultrastructural alterations are the first symptoms causing severe damages to yield and productivity. In this work, some physiological, biochemical, and molecular aspects of iron deficiency stress induced on the peach rootstock Mr.S 2/5 in vitro were studied. The aim was to demonstrate if in vitro culture can be used as a valid and fast method to evoke iron deficiency symptoms and to study plant responses to this kind of abiotic stress. Two different treatments were carried out and compared to a control (MS medium): plantlets grown on MS medium completely free of iron and on MS medium containing 1 mM potassium bicarbonate. After 10 and 20 days from the beginning of the treatments fresh and dry weight, chlorophyll, and carotenoids content were measured. Superoxide dismutase and catalase total activity was measured, and gene expression was analyzed by using etherologous probes (Sod1, Sod3, Sod4, and Cat1) obtained from maize. In the absence of iron or in the presence of bicarbonate Mr.S 2/5 plantlets showed a significant decrease in growth, as quantified by fresh and dry weight. The plantlets showed a severe chlorosis due to a reduction in chlorophyll and carotenoids concentration. Iron deficiency stress caused a reduction of the activity of catalase and superoxide dismutase, while inducing an increase in gene expression. Keywords: Antioxidant‐enzymesBicarbonateChlorosisGrowth Acknowledgments We thank Prof. J. G. Scandalios for kindly giving the probes Cat1, Sod1, Sod3, and Sod4 from maize and for his effective guidance in molecular analysis. We also acknowledge Dr. F. Cinelli for his helpful suggestions about bicarbonate treatments and Dr. M. Johnson for the revision of language. This research was supported by a grant from the Italian Ministry of University (MIUR‐A4002LS).