Design and Application of a Mevalonate‐Responsive Regulatory Protein

Abstract

Screening with a customized protein: The regulatory protein AraC in Escherichia coli was engineered to respond to the inducer mevalonate and used as an endogenous molecular reporter in library screening for improved inducer production (see picture; PBAD is the promoter). Mutants of a mevalonate-synthesis operon were expressed in E. coli, and a variant responsible for a fourfold increase in the mevalonate titer was isolated.