Determination of arsenic compounds by high‐pressure liquid chromatography–graphite furnace atomic absorption spectrometry and thermospray mass spectrometry

Abstract

Abstract Biologically important arsenic species such as arsenobetaine, arsenocholine iodide, tetramethylarsonium iodide, methylarsonic acid, and dimethylarsinic acid can be separated and quantitated by HPLC. The pH‐sensitive separations on a weak anion‐exchange column are described, as well as separations on a reverse‐phase column with the aid of tetrabutylammonium nitrate or heptanesulfonic acid as ion‐pairing agents. The thermospray mass spectra of these arsenicals in addition to those of sodium arsenate and an arsenosugar derivative are described. This technique is suitable for HPLC MS studies.