Deoxyribonucleic Acid Relatedness Between Enterobacter cloacae and Enterobacter amnigenus sp. nov.

Abstract

A deoxyribonucleic acid (DNA)-DNA hybridization study was carried out to determine the taxonomic position of a new group of enterobacters (group Ha, Gavini et al. [Ann.Microbiol.(Inst.Pasteur) 127B:317-335, 19761) previously studied by numerical taxonomy.The reassociation binding ratios with the group H3 strain ATCC 33072 ranged from 61 to 97%.With respect to DNA hybridization, this group, which represents a new species in the genus Enterobacter, Enterobacter amnigenus, is closely related (49 to 66%) to Enterobacter cloacae.A difference in genome size between E. amnigenus ATCC 33072 and E. cloacae Collection de l'Unit6 d'lhotoxicologie Microbienne (CUETM) 77-120 was observed after reciprocal binding: the genome of ATCC 33072 was 24% larger.E. amnigenus was defined from phenotypic and genetic data.The type strain of E.