Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System

TLDR

The authors correct mislabeled DNA ends in their figure and describe a simple, highly efficient CRISPR/Cas9 method for generating and detecting mutations in any Drosophila gene. RNA injection into Drosophila embryos yields up to 88 % mutagenesis efficiency, with germline transmission enabling mutant lines within a month. Corrected images are available online, and the full text is open access.

Abstract

(Cell Reports 4, 220–228; July 1, 2013) In the Graphical Abstract and Figure 1A, the 5’ and 3’ ends of the DNA were mislabeled. The upper strand that hybridizes with the sgRNA should read 3’ to 5’, and the lower strand that contains the sequence equivalent to the target site and the PAM sequence should read 5’ to 3’. The corrected images are provided here.Figure 1View Large Image Figure ViewerDownload Hi-res image Download (PPT) The authors apologize for this oversight. Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 SystemBassett et al.Cell ReportsJuly 1, 2013In BriefBassett, Liu, and colleagues present a simple and highly efficient method for generating and detecting mutations of any gene in Drosophila melanogaster through the use of the CRISPR/Cas9 system (clustered regularly interspaced palindromic repeats/CRISPR-associated). They show that injection of RNA into the Drosophila embryo can induce highly efficient mutagenesis of desired target genes in up to 88% of injected flies. These mutations can be transmitted through the germline, and this method offers the opportunity to generate a desired mutant fly line within one month. Full-Text PDF Open Access