Comparative Analysis of Methane-Oxidizing Archaea and Sulfate-Reducing Bacteria in Anoxic Marine Sediments

TLDR

Methane oxidation in anoxic marine sediments is believed to involve a consortium of methane‑oxidizing archaea and sulfate‑reducing bacteria. We compared rRNA gene surveys and lipid analyses of archaea and bacteria from multiple methane‑seep sites along the Californian continental margin. We found that ANME‑1 and ANME‑2 archaea, together with δ‑proteobacteria such as Desulfosarcina variabilis, dominate methane‑seep sediments, as shown by 13C‑depleted lipids, rDNA signatures, and FISH aggregations, and that additional bacterial groups likely participate in anaerobic methane oxidation.

Abstract

ABSTRACT The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several different sites on the Californian continental margin. Two distinct archaeal lineages (ANME-1 and ANME-2), peripherally related to the order Methanosarcinales , were consistently associated with methane seep marine sediments. The same sediments contained abundant 13 C-depleted archaeal lipids, indicating that one or both of these archaeal groups are members of anaerobic methane-oxidizing consortia. 13 C-depleted lipids and the signature 16S rDNAs for these archaeal groups were absent in nearby control sediments. Concurrent surveys of bacterial rDNAs revealed a predominance of δ-proteobacteria, in particular, close relatives of Desulfosarcina variabilis . Biomarker analyses of the same sediments showed bacterial fatty acids with strong 13 C depletion that are likely products of these sulfate-reducing bacteria. Consistent with these observations, whole-cell fluorescent in situ hybridization revealed aggregations of ANME-2 archaea and sulfate-reducing Desulfosarcina and Desulfococcus species. Additionally, the presence of abundant 13 C-depleted ether lipids, presumed to be of bacterial origin but unrelated to ether lipids of members of the order Desulfosarcinales , suggests the participation of additional bacterial groups in the methane-oxidizing process. Although the Desulfosarcinales and ANME-2 consortia appear to participate in the anaerobic oxidation of methane in marine sediments, our data suggest that other bacteria and archaea are also involved in methane oxidation in these environments.