Lab on a Chip · 2015 · 99 citations · 23 references
A self‑seeding microwell chip is introduced for the isolation and interrogation of single cells. The chip contains 6 400 microwells each with a 5 µm pore; a cell suspension is introduced, fluid flow drags a cell onto a pore, stops flow in that well while diverting remaining fluid to the next well, and after fluorescence identification, cells are isolated by punching the bottom together with the cell. The system achieves fast, efficient single‑cell distribution and a >70 % recovery rate with 100 % specificity, as verified by genetic analysis.
A self-seeding microwell chip is introduced for the isolation and interrogation of single cells. A cell suspension is transferred to a microwell chip containing 6400 microwells, each microwell with a single 5 μm pore in the bottom. The fluid enters the microwell and drags a cell onto the pore. After a cell has landed onto the pore, it will stop the fluid flow through this microwell. The remaining fluid and cells will be diverted to the next available microwell. This results in a fast and efficient distribution of single cells in individual microwells. After identification by fluorescence microscopy, the cells of interest are isolated from the microwell by punching the bottom together with the cell. The overall single cell recovery of seeding followed by isolation of the single cell, is >70% with a specificity of 100% as confirmed by the genetic make-up of the isolated cells.
23
A microfabricated fluorescence-activated cell sorter
Anne Y. Fu, Charles Spence, Axel Scherer et al. · Nature Biotechnology · 1999 · 872 citations
Michael Craig Miller, Gerald V. Doyle, Leon W.M.M. Terstappen · Journal of Oncology · 2009 · 753 citations · Full text
Breast Oncology, Ctc Definition, Circulating Tumor Cells +18