Genetic Mapping of <i>aro, pyr</i>, and <i>pur</i> Markers in <i>Klebsiella pneumoniae</i>

Abstract

ABSTRACT Various aro, pyr, pur , and two other markers were located on the genetic linkage map of Klebsiella pneumoniae by employing a mating system in which genetic material was transferred by conjugation. These markers are widely scattered on the chromosome, and their locations were found to be similar to those of Escherichia coli . Biochemical and genetic investigations suggested that in K. pneumoniae , as well as in E. coli , the biosynthesis of 3‐deoxy‐D‐arabino‐heptulosonic acid‐7‐phosphate was catalyzed by 3 isozymes. A revised genetic linkage map of K. pneumoniae including these markers has been presented.