Life SciencesBiochemistryMicrobiologyBiocatalysisEnzymologyMycologyEnzyme DiscoveryEnzyme IsolationEnzyme ProductionFungal BiochemistryFungal MetabolismMicrobial Enzyme ProductionAmylase ProductionFungal BiocatalysisFungal Enzyme ProductionFungal Enzyme SecretionFungal EnzymologyInternal Transcribed Spacer SequencingOptimal Ph Determination
Fungi are well known for their ability to excrete enzymes into the environment. The fungal isolate FSS60 was the best amylase producer among one hundred and thirty-six isolates obtained from Syrian soils and tested for amylase production. According to the sequence of the internal transcribed spacer (ITS) rDNA gene, the isolate was identified as Aspergillus flavus. Optimal initial pH for amylase production was found to be 9.0. The enzyme was optimally active at 50 degrees C and pH 5.0.
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