Concepedia

A New Reversion Assay for Measuring All Possible Base Pair Substitutions in Saccharomyces cerevisiae

Teresa-Marie Williams, Rebecca M Fabbri, Jason Reeves, Gray F. Crouse

Genetics · 2005 · 15 citations · 23 references

DOIFull text

Open access

Concepts

Abstract

A TRP5-based reversion system that allows the rates of all possible base pair substitutions to be measured when the TRP5 locus is in both orientations relative to a defined origin of replication has been developed. This system should be useful for a wide variety of mutation and repair studies in yeast.

References

23

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

Julie Thompson, Desmond G. Higgins, Toby J. Gibson · Nucleic Acids Research · 1994 · 64.9K citations · Full text

, , +14

A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions.

Claire G. Cupples, Jeffrey H Miller · Proceedings of the National Academy of Sciences · 1989 · 454 citations · Full text

, , +17

In vivo site-directed mutagenesis using oligonucleotides

Francesca Storici, L. Kevin Lewis, Michael A. Resnick · Nature Biotechnology · 2001 · 338 citations

, , +9

Yeast gene TRP5: structure, function, regulation.

H Zalkin, Charles Yanofsky · Journal of Biological Chemistry · 1982 · 238 citations · Full text

, , +10

Inactivation of OGG1 increases the incidence of G · C→T · A transversions in Saccharomyces cerevisiae : evidence for endogenous oxidative damage to DNA in eukaryotic cells

Dominique Thomas, A. D. Scot, Régine Barbey et al. · Molecular and General Genetics MGG · 1997 · 221 citations

, , +12