Molecular Microbiology · 2004 · 161 citations · 24 references
Life SciencesMicrobiologyMolecular BiologyDna RepairMicrobial GeneticsRadiobiologyDna Double-strand Break RepairDna LigaseDna Strand BreaksDouble-strand Break RepairNon-homologous End JoiningRadiation ResistanceDna Double-strand BreaksDna Repair MechanismsDna Strand Break RepairDouble-strand Dna BreaksEscherichia ColiRadiation Resistance MechanismsRadiation-resistant MicrobiologyRepair Of Ionizing Radiation-induced Breaks
The extraordinary radiation resistance of Deinococcus radiodurans results from the efficient capacity of the bacterium to repair DNA double-strand breaks. By analysing the DNA damage repair-deficient mutant, KH311, a unique radiation-inducible gene (designated pprA) responsible for loss of radiation resistance was identified. Investigations in vitro showed that the gene product of pprA (PprA) preferentially bound to double-stranded DNA carrying strand breaks, inhibited Escherichia coli exonuclease III activity, and stimulated the DNA end-joining reaction catalysed by ATP-dependent and NAD-dependent DNA ligases. These results suggest that D. radiodurans has a radiation-induced non-homologous end-joining repair mechanism in which PprA plays a critical role.
24
Genome Sequence of the Radioresistant Bacterium <i>Deinococcus radiodurans</i> R1
Owen White, Jonathan A. Eisen, John F. Heidelberg et al. · Science · 1999 · 967 citations
The genome sequence of the extreme thermophile Thermus thermophilus
Anke Henne, Holger Brüggemann, Carsten Raasch et al. · Nature Biotechnology · 2004 · 391 citations · Full text
Identification of a DNA Nonhomologous End-Joining Complex in Bacteria
Geoffrey R. Weller, Boris Kysela, Rajat Roy et al. · Science · 2002 · 336 citations · Full text