Angewandte Chemie International Edition · 2010 · 72 citations · 19 references
Life SciencesBiochemistryBiotechnologyMolecular ImagingNanomaterialsProtein EngineeringDrug EncapsulationFluorescence SpectroscopyNanomedicineNanoparticle FormulationsProtein DeliveryProtein-based NanomaterialsFluorescent ProbesGreen Fluorescent Protein ReportersNanoparticle-based ProbesNanoparticlesProtein Delivery NanoparticlesProtein FluorescenceProtein-based NanoparticlesSilica-based Nanoparticles
Si′l vous plait? A facile and general method has been developed to encapsulate polyhistidine-tagged proteins in silica nanoparticles (NPs; gray, see picture) using calcium ions (yellow). The enhanced green fluorescence protein (EGFP) encapsulated in the silica NPs shows a substantial increase in fluorescence intensity and stability against denaturants, protease, and heat.
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