Recent developments in cryopreservation of stallion semen with special emphasis on thawing procedure using thermal hysteresis proteins

Abstract

While the first attempt to cool and preserve stallion semen was done 200 years ago (by Spallazani) and the first pregnancy achieved with frozen-thawes stallion semen almost 40 years ago (Barker & Gandier, 1957), frozen stallion semen has not been used widely, mainly because of its association with low fertility.