Single‐Cell Multiplex Gene Detection and Sequencing with Microfluidically Generated Agarose Emulsions

Abstract

Solitary confinement: The described approach enabled high-throughput DNA preparation followed by multiplex emulsion PCR for the detection and sequencing of genetic targets (see picture). No droplet sorting was required, and single-cell segregation was maintained throughout owing to the incorporation of primer-functionalized beads as amplicon substrates. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.