Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter

High-resolution (1.5 Å) crystal structure of phospholipase C from Bacillus cereus

Edward Hough, Lars Kristian Hansen, Bjørn Birknes et al. · Nature · 1989 · 454 citations

Crystal Structure, Biochemistry, Protein X-ray Crystallography +7

Promoter library designed for fine-tuned gene expression in Pichia pastoris

Franz Stefan Hartner, Claudia Rüth, David S. Langenegger et al. · Nucleic Acids Research · 2008 · 306 citations · Full text

Phospholipase C assay using p-nitrophenylphosphorylcholine together with sorbitol and its application to studying the metal and detergent requirement of the enzyme

Susumu Kurioka, Makoto Matsuda · Analytical Biochemistry · 1976 · 280 citations

Biochemistry, Detergent Requirement, Medicine +4

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

Florian Krainer, Christian Dietzsch, Tanja Hajek et al. · Microbial Cell Factories · 2012 · 203 citations · Full text

Fermentation Development of Recombinant <i>Pichia pastoris</i> Expressing the Heterologous Gene: Bovine Lysozyme

R. A. Brierley, Christopher M. Bussineau, Ryszard Kosson et al. · Annals of the New York Academy of Sciences · 1990 · 163 citations

Biosynthesis, Biomanufacturing, Engineering +11