Concepedia

Multicolor Labeling of Living‐Virus Particles in Live Cells

Peng Zhou, Zhenhua Zheng, Wen Lu, Fuxian Zhang, Zhenfeng Zhang, Dai‐Wen Pang, Bin Hu, Zhike He, Hanzhong Wang

Angewandte Chemie International Edition · 2011 · 34 citations · 23 references

DOI

Concepts

Abstract

Dual-color virus particles were obtained by labeling a glycoprotein (GP64) on the surface of a baculovirus with a green fluorescent protein (GFP) and labeling the nucleic acid (dsDNA) at the same time with the red fluorescent [Ru(phen)2(dppz)]2+ complex during the viral replication in host cells, by using an in vivo virus self-assembly system. This labeling strategy does not affect the viral infectivity.

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