Cytochrome P450-mediated hydroxylation is required for polyketide macrolactonization in stambomycin biosynthesis

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15.2K citations · Full text

Life Sciences, Genetics, Molecular Genetics +17

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.3K citations

Life Sciences, Genetics, Microbiology +12

PCR-targeted <i>Streptomyces</i> gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin

Bertolt Gust, Gregory L. Challis, Kay Fowler et al. · Proceedings of the National Academy of Sciences · 2003 · 1.4K citations

Life Sciences, Biotechnology, Microbiology +14

Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector

Douglas J. MacNeil, Keith M. Gewain, Carolyn L. Ruby et al. · Gene · 1992 · 761 citations

Life Sciences, Biotechnology, Microbiology +9

Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in <i>Streptomyces</i> <i>avermitilis</i>

Haruo Ikeda, Tomoko Nonomiya, Masayo Usami et al. · Proceedings of the National Academy of Sciences · 1999 · 349 citations · Full text

Life Sciences, Microbiology, Molecular Biology +12